M13 Bacteriophage G8P Capsid 抗体 (Biotin)
This anti- antibody is a 小鼠 单克隆 antibody detecting in WB 和 EIA. Suitable for Bacteriophage M13.
Quick Overview for M13 Bacteriophage G8P Capsid 抗体 (Biotin) (ABIN125965)
抗原
M13 Bacteriophage G8P Capsid
适用
Bacteriophage M13
宿主
小鼠
克隆类型
单克隆
标记
Biotin
应用范围
Western Blotting (WB), Enzyme Immunoassay (EIA)
克隆位点
B62-FE2
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特异性
- B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT (cf. Kneissel et al.)
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纯化方法
- Affinity Chromatography on Protein A
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免疫原
- fd phages from E. coli F+ strain (JM109)
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亚型
- IgG2b
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anti-M13 Bacteriophage G8P Capsid antibody
适用: Bacteriophage M13 WB, FACS, ICC, ELISA 宿主: 小鼠 Monoclonal RL-ph1 unconjugated
anti-M13 Bacteriophage G8P Capsid antibody适用: Bacteriophage M13 WB, FACS, ICC, ELISA, IHC 宿主: 小鼠 Monoclonal LRL-ph2 unconjugated
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应用备注
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Phage Display: Immunoassays for the identification of recombinant antigen- orantibody-phages. Detection limit: 10e7 phage particles. Working dilution for ELISA: 1/5,000-1/10,000. Immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C up to one month or (in aliquots) at -20 °C for longer.
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- M13 Bacteriophage G8P Capsid
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物质类
- Phage Protein
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背景
- M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest.Synonyms: Coat protein B, Gene 8 protein, Major coat protein
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基因ID
- 927333
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NCBI登录号
- NP_510890
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UniProt
- P69541
抗原
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