PRDX6-PS2 抗体 (C-Term)
There are 2+ publications for this product available. The 兔 多克隆 anti-PRDX6-PS2 antibody is suitable to detect PRDX6-PS2 in samples from Arabidopsis thaliana. It has been validated for ELISA 和 WB.
(2 references)
产品编号 ABIN125822
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Quick Overview for PRDX6-PS2 抗体 (C-Term) (ABIN125822)
抗原
PRDX6-PS2
(Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))
适用
Arabidopsis thaliana
宿主
兔
克隆类型
多克隆
应用范围
ELISA, Western Blotting (WB)
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抗原表位
- C-Term
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原理
- Anti-GPX | Glutathione peroxidase (chloroplastic)
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交叉反应 （详细）
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Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
纯化方法
- Serum
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免疫原
- thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of Arabidopsis thaliana GPX7 accession At4g31870 has been used for antibody production
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应用备注
- 1 : 1, 1 : 2, 1 :4, 1 :8, 1 : 16 (ELISA), 1 : 5000 (WB)
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说明
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This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms). Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods." in: Journal of experimental botany, Vol. 64, Issue 12, pp. 3843-54, (2013) (PubMed).
: "Decarbonylated cyclophilin A Cpr1 protein protects Saccharomyces cerevisiae KNU5377Y when exposed to stress induced by menadione." in: Cell stress & chaperones, (2010) (PubMed).
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: "Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods." in: Journal of experimental botany, Vol. 64, Issue 12, pp. 3843-54, (2013) (PubMed).
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- PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))
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别名
- GPX
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背景
- GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX.
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分子量
- 25 | 20 kDa
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基因ID
- 829316
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UniProt
- Q9SZ54
抗原
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