Orf1 (N. Meningitidis) 抗体
Cited in 1 publication.
The 兔 多克隆 anti-Orf1 (N. Meningitidis) antibody (ABIN125757) specifically detects Orf1 (N. Meningitidis) in WB.
The antibody is reactive with Neisseria meningitidis samples.
1 image
(1 reference)
产品编号 ABIN125757
发货至:
中国
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Quick Overview for Orf1 (N. Meningitidis) 抗体 (ABIN125757)
抗原
Orf1 (N. Meningitidis)
适用
Neisseria meningitidis
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB)
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原理
- Anti-Orf1 (N. meningitidis) Purified
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交叉反应 （详细）
- Neisseria meningitidis
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产品特性
- The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.
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应用备注
- Western blotting: Recommended dilution 0.5-1 μg/mL, reducing conditions.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC." in: The Journal of biological chemistry, Vol. 280, Issue 5, pp. 3251-8, (2005) (PubMed).
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- Orf1 (N. Meningitidis)
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背景
- Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.,FrpD
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基因ID
- 35
抗原
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