ITGAL 抗体
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北京 101111
Quick Overview for ITGAL 抗体 (ABIN125722)
抗原
See all ITGAL 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu CD11a Purified Azide Free
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特异性
- The antibody MEM-83 reacts with an extracellular epitope of CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
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交叉反应 （详细）
- Human
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Human peripheral blood lymphocytes
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亚型
- IgG1
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anti-Integrin alpha-L (ITGAL) (AA 26-306) antibody
ITGAL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha-L (ITGAL) (AA 26-194) antibodyITGAL 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 5G7C5 unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人 FACS, IP, IHC 宿主: 小鼠 Monoclonal 38 unconjugated
anti-Integrin alpha-L (ITGAL) (AA 201-500) antibodyITGAL 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 小鼠 FACS, IP, IHC (fro) 宿主: 大鼠 Monoclonal M17-4 unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-25 unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人 FACS, IP, Func 宿主: 小鼠 Monoclonal MEM-25 unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人 FACS, IP, Func 宿主: 小鼠 Monoclonal MEM-25 unconjugated
anti-Integrin alpha-L (ITGAL) antibody (Biotin)ITGAL 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-25 Biotin
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应用备注
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Functional application: The antibody MEM-83 directly induces the binding of T cells to purified ICAM-1. Using an in vitro-translated CDlla cDNA deletion series, the MEM-83 activation epitope was mapped to the "I" domain of the LFA-1 alpha subunit. The studies have therefore identified a novel LFA-1 activation epitope mapping to the I domain of LFA-1, which could play a role in the regulation of LFA-1 binding to ICAM-1.
Flow cytometry: Recommended dilution: 1 μg/mL. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4
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储存液
- Azide free
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3." in: The Journal of cell biology, Vol. 126, Issue 2, pp. 529-37, (1994) (PubMed).
: "A novel LFA-1 activation epitope maps to the I domain." in: The Journal of cell biology, Vol. 120, Issue 6, pp. 1519-27, (1993) (PubMed).
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: "Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3." in: The Journal of cell biology, Vol. 126, Issue 2, pp. 529-37, (1994) (PubMed).
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- ITGAL (Integrin alpha-L (ITGAL))
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别名
- CD11a
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背景
- Integrin subunit alpha L,CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.,LFA-1, LFA1A, ITGAL
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基因ID
- 3683
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UniProt
- P20701
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途径
- Activated T Cell Proliferation, Integrin Complex
抗原
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