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Cytokeratin 19 抗体 (Biotin)

This anti-Cytokeratin 19 antibody is a 小鼠 单克隆 antibody detecting Cytokeratin 19 in WB, FACS, ELISA, IHC (p), ICC 和 IP. Suitable for 人. This Primary Antibody has been cited in 2+ publications.
产品编号 ABIN125676
发货至: 中国

Quick Overview for Cytokeratin 19 抗体 (Biotin) (ABIN125676)

抗原

See all Cytokeratin 19 (KRT19) 抗体
Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Cytokeratin 19 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

克隆位点

A53-B-A2
  • 原理

    Anti-Cytokeratin 19 Biotin

    特异性

    The mouse monoclonal antibody A53-B/A2 recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes, it is often co-expressed with cytokeratin 7.

    交叉反应 (详细)

    Human

    纯化方法

    Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

    免疫原

    MCF-7 human breast adenocarcinoma cell line

    亚型

    IgG2a
  • 应用备注

    Flow cytometry: Recommended dilution: 1-5 μg/mL, intracellular staining.
    Western blotting: Recommended dilution: 1-2 μg/mL.

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Do not freeze.
    Avoid prolonged exposure to light.

    储存条件

    4 °C

    储存方法

    Store at 2-8°C. Do not freeze.
  • Kasper, Moll, Stosiek, Karsten: "Patterns of cytokeratin and vimentin expression in the human eye." in: Histochemistry, Vol. 89, Issue 4, pp. 369-77, (1988) (PubMed).

    Karsten, Papsdorf, Roloff, Stolley, Abel, Walther, Weiss: "Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion." in: European journal of cancer & clinical oncology, Vol. 21, Issue 6, pp. 733-40, (1985) (PubMed).

  • 抗原

    Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

    别名

    Cytokeratin 19

    背景

    Keratin 19,Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K19, CK19, CYK19, KRT19, K1CS

    基因ID

    3880

    UniProt

    P08727
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