Immunohistochemistry on paraffin embedded sections: 3,5 μg/mLAntigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove fromheat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween(TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.7, containing 0.01 % sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-80 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
PAK6, a PAK (STE20) type protein kinase, contains a N-terminal Cdc42/Rac interactive binding (CRIB) motif and a C-terminal kinase domain. The protein has been shown to bind to GTP-Cdc42 and GTP-Rac, however, in contrast to most PAKs, kinase activity was not stimulated by Cdc42 or Rac, but could be stimulated by androgen receptor (AR) binding. PAK6 protein also has been shown to bind to estrogen receptor alpha. PAK6 also has been called P21-Activated Kinase 5 (PAK5), however, PAK5 is a distinct gene found on chromosome 20 p12.2. PAK6 expression has been documented in normal human brain, breast, prostate and testis. ESTs have been isolated from several tissue libraries, including normal brain and testis and cancerous cervix and smooth muscle.Synonyms: PAK-5, PAK-6, PAK5, Serine/threonine-protein kinase PAK 6, p21-activated kinase 6