Western Blot. Immunocytochemistry. Immunohistochemistry with formalin fixed paraffin embedded tissues: 5-10 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS containing 0.09 % Sodium Azide as preservative
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 to -80 °C forlonger. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
The Metabotropic Glutamate Receptor GRM2 binds L-glutamate and has been linked to the inhibition of the cyclic AMP signaling cascade. GRM2 is involved in mediating the suppression of neurotransmission and in synaptogenesis and synaptic stabilization. Glutamatergic neurotransmission plays a role in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. GRM2 has been reported in various regions of the human and animal brain, as well as in rat eye and spinal cord. ESTs have been isolated from brain and testis libraries. Caution: GLUR2 refers to Ionotropic Glutamate Receptor 2, not to Metabotropic Glutamate Receptor 2 (mGLUR2).Synonyms: GPRC1B, Metabotropic glutamate receptor 2