Immunohistochemistry on paraffin embedded sections: 5 μg/mL. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS containing 0.09 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
CCR1, a Chemokine Receptor, affects stem cell proliferation and mediates systemic inflammatory responses. This receptor is stimulated by the beta chemokines RANTES, MCP-1, and MIP-1 alpha. Disruption of CCR1 enhances protection from pulmonary inflammation and reduces the development of respiratory distress syndrome in rat. CCR1 is widely expressed, particularly in hematopoietic cells from blood, vessels, and bone marrow. This receptor has also been reported to be expressed in lung, brain, placenta, heart, skeletal muscle, and kidney. ESTs have been isolated from blood, kidney, liver/spleen, lung, placenta, uterus, and vessel libraries.Synonyms: C-C CKR-1, C-C chemokine receptor type 1, CC-CKR-1, CCR-1, CMKBR1, CMKR1, HM145, LD78 receptor, MIP1 alpha Receptor, Macrophage inflammatory protein 1-alpha receptor, RANTES-R, SCYAR1