beta-2 Microglobulin 抗体
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- 抗原 See all beta-2 Microglobulin (B2M) 抗体
- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
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适用
- 人, Pig
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This beta-2 Microglobulin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 特异性
- The antibody B2M-02 reacts with beta2-microglobulin (b2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin.
- 纯化方法
- Protein A chromatography
- 纯度
- > 95 % (by SDS-PAGE).
- 免疫原
- Purified isolated human beta2-microglobulin
- 克隆位点
- B2M-02
- 亚型
- IgG1
- Top Product
- Discover our top product B2M Primary Antibody
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- 应用备注
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Flow cytometry (1 μg/mL): use PBL cell line as positive control. Negative control: DAUDI human lymphoma cell line, erythrocytes. Western blot (2 - 4 μg/mL): use U937 human lymphoma cell line as positive control. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Application note: Both reducing and non-reducing conditions. Reducing and non-reducing conditions. ELISA. Immunohistochemistry (Paraffin): Recommended dilution: 5 m g/mL, Positive tissue: liver
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 15 mM sodium azide, approx. pH 7.4
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. - 有效期
- 12 months
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- 抗原
- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
- 别名
- beta-2-Microglobulin (B2M 产品)
- 别名
- Ly-m11 antibody, beta2-m antibody, beta2m antibody, bwm antibody, wu:fa94c05 antibody, wu:fb10a09 antibody, b2m-W01 antibody, b2m-W03 antibody, b2m-Z01 antibody, b2m-Z02 antibody, b2m-Z03 antibody, B2M antibody, b2m antibody, B2MG antibody, wu:fb66f11 antibody, wu:fj01f08 antibody, zgc:64161 antibody, b-2-m antibody, beta-2-microglobulin antibody, beta-2 microglobulin antibody, beta-2-microglobulin S homeolog antibody, beta-2-microglobulin, like antibody, B2M antibody, B2m antibody, b2m antibody, b2m.S antibody, b2ml antibody
- 背景
- B2M is a 12 kDa Ig like glycoprotein. B2M non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.Synonyms: B2M, Beta 2 microglobulin, Beta-2 microglobulin
- 基因ID
- 567
- UniProt
- P61769
- 途径
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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