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CD235a/GYPA 抗体 (N-Term)

This anti-CD235a/GYPA antibody is a 小鼠 单克隆 antibody detecting CD235a/GYPA in FACS, IHC (p), IHC (fro), AGG 和 H. Suitable for 人. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN120751
发货至: 中国

Quick Overview for CD235a/GYPA 抗体 (N-Term) (ABIN120751)

抗原

See all CD235a/GYPA (GYPA) 抗体
CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

适用

  • 168
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宿主

  • 101
  • 72
  • 10
  • 1
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  • 1
小鼠

克隆类型

  • 109
  • 76
单克隆

标记

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This CD235a/GYPA antibody is un-conjugated

应用范围

  • 99
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Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Aggregation (AGG), Haemagglutination (H)

克隆位点

HIR2
  • 抗原表位

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    N-Term

    特异性

    This antibody clone HIR2 recognizes N-terminal portion of glycophorin A (and weakly of glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.

    纯化方法

    Protein G chromatography

    免疫原

    Synthetic peptide (Human, N-terminal)

    亚型

    IgG2b
  • 应用备注

    Flow cytometry: This HIR2 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes and cell agglutination assay and can be used at approximately 0.1 μg per million cells. Immunohistochemistry (frozen sections). Agglutination: Clone HIR2 agglutinates untreated RBCs but it failes to agglutinate papain- treated cells.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS with 15 mM sodium azide, approx. pH 7.4

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf Life: one year from despatch.

    有效期

    12 months
  • Moles, Galiano, Gomes, Quiliano, Teixeira, Aldana, Gomes, Fernàndez-Busquets: "ImmunoPEGliposomes for the targeted delivery of novel lipophilic drugs to red blood cells in a falciparum malaria murine model." in: Biomaterials, Vol. 145, pp. 178-191, (2018) (PubMed).

  • 抗原

    CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

    别名

    CD235a / GYPA

    背景

    CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.Synonyms: GPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha

    基因ID

    2993

    UniProt

    P02724

    途径

    Maintenance of Protein Location
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