Vimentin 抗体
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北京 101111
Quick Overview for Vimentin 抗体 (ABIN120733)
抗原
See all Vimentin (VIM) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The antibody reacts with Vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types in all species (recognized epitope conserved within all species). Cross-reactivity was found with smooth muscle Desmin.
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纯化方法
- Precipitation Methods
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免疫原
- Pellet of pig brain cold stable proteins after depolymerization of microtubules
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亚型
- IgM
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anti-Vimentin (VIM) (AA 371-466) antibody
VIM 适用: 人, 大鼠, 小鼠, Pig, Cow, 猴 WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) (AA 435-466), (C-Term) antibodyVIM 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) antibodyKO Validated VIM 适用: 人 WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) (AA 1-200) antibodyVIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人, 大鼠, 小鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人, 小鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB41034 unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Vimentin (VIM) (AA 41-140) antibodyVIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated
anti-Vimentin (VIM) (AA 210-466) antibodyVIM 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D7 unconjugated
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应用备注
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Western Blotting. Immunocytochemistry: Staining technique: RBL rat basophilic leukemia cell line:(a) Fix cells for 10 min in methanol at - 20 °C and for 6 min in acetone at -20 °C, (b) Fix cells directly in methanol for 10 min at -20 °C or in acetone for 10 min at -20 °C. Incubation: 45 min RT, Positive control: 3T3 mouse Swiss albino fibroblast cell line RBL rat basophilic leukemia cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS , pH ~7.4, 15 mM Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Store the antibody at 2 - 8 °C. Do not freeze!
Shelf Life: one year from despatch. -
有效期
- 12 months
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: "Differential proteome analysis of human gliomas stratified for loss of heterozygosity on chromosomal arms 1p and 19q." in: Neuro-oncology, Vol. 12, Issue 3, pp. 243-56, (2010) (PubMed).
: "Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture." in: Journal of biomedical materials research, Vol. 49, Issue 3, pp. 369-79, (2000) (PubMed).
: "Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions." in: Biomaterials, Vol. 21, Issue 11, pp. 1173-9, (2000) (PubMed).
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- Vimentin (VIM)
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别名
- Vimentin
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背景
- Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin. Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.Synonyms: VIM
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基因ID
- 7431
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UniProt
- P08670
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途径
- Caspase Cascade in Apoptosis
抗原
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