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LYN 抗体 (AA 8-238)

Name: LYN 抗体 (AA 8-238)
SKU: ABIN120725
Availability: OutOfStock

This anti-LYN antibody is a 小鼠单克隆 antibody detecting LYN in WB, IP 和 IF. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 3+ publications.

(3 references)

产品编号 ABIN120725

发货至: 中国

Quick Overview for LYN 抗体 (AA 8-238) (ABIN120725)

抗原

See all LYN 抗体

LYN (V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN))

适用

人, 小鼠, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This LYN antibody is un-conjugated

应用范围

Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

克隆位点

LYN-01

Discover our top product LYN Primary Antibody

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AA 8-238

特异性

The antibody reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues.

纯化方法

Protein A affinity chromatography

免疫原

Bacterially expressed recombinant fragment of human Lyn (aa 8 - 238)

亚型

IgG1
anti-V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) (pTyr507) antibody
LYN 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) (C-Term) antibody
LYN 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) (Center) antibody
LYN 适用: 人 WB, IP, IHC (p), ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) (AA 229-263) antibody
LYN 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) (AA 32-62) antibody
LYN 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) (AA 470-501) antibody
LYN 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) (pTyr397) antibody
LYN 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) (pTyr508) antibody
LYN 适用: 人, 小鼠, 大鼠, Cow, Pig, 小鸡, 犬, 豚鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) (AA 229-263) antibody
LYN 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB50588 unconjugated

anti-V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) (AA 39-58) antibody
LYN 适用: 人, 小鼠, 大鼠 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal unconjugated

应用备注

Immunoprecipitation. Immunocytochemistry. Western Blotting: Recommended dilution: 1-2 μg/mL, 60 min. Positive control: RBL rat basophilic leukemia cell line JURKAT human peripheral blood leukemia T-cell line A-431 human epidermoid carcinoma cell line U-937 human histiocytic lymphoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate (1:1) with non- reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (12 % separating gel).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS, pH ~7.4, 15 mM Sodium Azide

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

有效期

12 months
Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).

Kovárová, Tolar, Arudchandran, Dráberová, Rivera, Dráber: "Structure-function analysis of Lyn kinase association with lipid rafts and initiation of early signaling events after Fcepsilon receptor I aggregation." in: Molecular and cellular biology, Vol. 21, Issue 24, pp. 8318-28, (2001) (PubMed).

Amoui, Dráberová, Tolar, Dráber: "Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors." in: European journal of immunology, Vol. 27, Issue 1, pp. 321-8, (1997) (PubMed).
抗原

LYN (V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN))

别名

LYN

背景

Lyn (also known as p53/56 Lyn) is a membrane associated protein tyrosine kinase (PTK) mostly expressed in hemopoietic cells which is important in cellular signaling. It contains an SH2 and SH3 domain and has been found to be cleaved after activation of caspases in apoptosis. A member of the Src family of PTKs, there are two known isoforms for Lyn which plays an indispensable role in the Fc epsilon RI (Fcer1) and the B cell IgM receptor signaling pathway and is essential for Syk activation and Lat phosphorylation after Fcer1 aggregation and can also phosphorylate Tec on multiple residues. Lyn can also be regulated by IL2 and IL3. Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI).Synonyms: Tyrosine-protein kinase Lyn

基因ID

4067

UniProt

P07948

途径

Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling, Integrin Complex, BCR Signaling