MHC Class I RT1.Aa alpha-Chain (RT1.AA) 抗体
Cited in 1 publication.
The 小鼠 单克隆 anti-MHC Class I RT1.Aa alpha-Chain antibody (Clone OX-18) (ABIN120723) specifically detects MHC Class I RT1.Aa alpha-Chain in FACS, IHC (fro) 和 IP.
The antibody is reactive with 大鼠 samples.
(1 reference)
产品编号 ABIN120723
发货至:
中国
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Quick Overview for MHC Class I RT1.Aa alpha-Chain (RT1.AA) 抗体 (ABIN120723)
抗原
See all MHC Class I RT1.Aa alpha-Chain (RT1.AA) 抗体
MHC Class I RT1.Aa alpha-Chain (RT1.AA)
适用
大鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
克隆位点
OX-18
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纯化方法
- Affinity chromatography on Protein G
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免疫原
- Rat spleen glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line
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亚型
- IgG1
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anti-MHC Class I RT1.Aa alpha-Chain (RT1.AA) antibody
RT1.AA 适用: 大鼠 FACS, IHC (fro), IP 宿主: 小鼠 Monoclonal OX-18 unconjugated
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应用备注
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ELISA. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections: Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. -
有效期
- 12 months
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: "[Aortic root replacement and pulmonary vein isolation, report of a case]" in: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 2, pp. 153-6, (2008) (PubMed).
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- MHC Class I RT1.Aa alpha-Chain (RT1.AA)
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别名
- MHC Class I (RT1Aa)
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背景
- MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: RT1 class I histocompatibility antigen AA alpha chain
抗原
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