MHC Class I RT1.Aa alpha-Chain (RT1.AA) 抗体
This 小鼠 单克隆 antibody specifically detects MHC Class I RT1.Aa alpha-Chain in FACS, IHC (fro) 和 IP. It exhibits reactivity toward 大鼠.
产品编号 ABIN120721
发货至:
中国
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Quick Overview for MHC Class I RT1.Aa alpha-Chain (RT1.AA) 抗体 (ABIN120721)
抗原
See all MHC Class I RT1.Aa alpha-Chain (RT1.AA) 抗体
MHC Class I RT1.Aa alpha-Chain (RT1.AA)
适用
大鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
克隆位点
OX-18
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纯化方法
- Protein G affinity chromatography
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免疫原
- Rat spleen cell glycoprotein
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亚型
- IgG1
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anti-MHC Class I RT1.Aa alpha-Chain (RT1.AA) antibody
RT1.AA 适用: 大鼠 FACS, IHC (fro), IP 宿主: 小鼠 Monoclonal OX-18 unconjugated
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应用备注
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Flow cytometry: 1:50-1:100, use 10 μL of the suggested working dilution to label 10^6 cells. Immunohistochemistry on frozen sections: Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde. Not suitable on paraffin embedded sections. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS, pH 7.4, containing 0.09 % sodium azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody at 2-8 °C for up to one month or at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: one year from despatch. -
有效期
- 12 months
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- MHC Class I RT1.Aa alpha-Chain (RT1.AA)
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别名
- MHC Class I (RT1Aa)
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背景
- MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: RT1 class I histocompatibility antigen AA alpha chain
抗原
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