KIF5B 抗体
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北京 101111
Quick Overview for KIF5B 抗体 (ABIN120716)
抗原
See all KIF5B 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The antibody KN-03 recognizes heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-03 well recognizes kinesin bound to taxol-stabilized microtubules.
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纯化方法
- Thiophilic Adsorption Affinity Chromatography
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免疫原
- Enriched fraction of porcine brain kinesin
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亚型
- IgM
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应用备注
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Immunocytochemistry. Western blot (kinesin-enriched presoarations).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH ~7.4, 15 mM Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Store at 2 - 8 °C. Do not freeze.
Shelf Life: one year from despatch. -
有效期
- 12 months
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- KIF5B (Kinesin Family Member 5B (KIF5B))
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别名
- KIF5B
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背景
- Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.Synonyms: Conventional kinesin heavy chain, KIF5B, KNS, KNS1, Kinesin-1 heavy chain, UKHC, Ubiquitous kinesin heavy chain
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基因ID
- 3799
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UniProt
- P33176
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途径
- Peptide Hormone Metabolism, Ribonucleoprotein Complex Subunit Organization
抗原
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