alpha Fetoprotein 抗体
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北京 101111
Quick Overview for alpha Fetoprotein 抗体 (ABIN120681)
抗原
See all alpha Fetoprotein (AFP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The antibody reacts with 70 kDa alpha-Fetoprotein (AFP).
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Precipitation Methods
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免疫原
- Purified alpha-Fetoprotein
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亚型
- IgG1
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anti-alpha-Fetoprotein (AFP) (AA 500-609) antibody
AFP 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 1G7 unconjugated
anti-alpha-Fetoprotein (AFP) (AA 399-605) antibodyAFP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) antibodyAFP 适用: 人, 犬, 猴 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 2A9 unconjugated
anti-alpha-Fetoprotein (AFP) (AA 31-171) antibodyAFP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) (AA 409-611) antibodyAFP 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) antibodyAFP 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (p) 宿主: 小鼠 Monoclonal A2 unconjugated
anti-alpha-Fetoprotein (AFP) (C-Term) antibodyAFP 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) (AA 19-397) antibodyAFP 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal OTI2F11 unconjugated
anti-alpha-Fetoprotein (AFP) (AA 281-609) antibodyAFP 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 1087CT8-3-5 unconjugated
anti-alpha-Fetoprotein (AFP) (AA 234-380) antibodyAFP 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunoprecipitation: Interaction of the antibody with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions. ELISA: The antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-Fetoprotein in combination with clone AFP-11 (cat. no. ABIN120683). Western Blotting: Positive material: Hep G2 human hepatocellular carcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing/reducing Laemmli SDS-PAGE sample buffer. Application note: Both non-reducing and reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. -
有效期
- 12 months
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- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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背景
- AFP is an oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP
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基因ID
- 174
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UniProt
- P02771
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途径
- C21-Steroid Hormone Metabolic Process
抗原
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