CD22 抗体
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- 抗原 See all CD22 抗体
- CD22 (CD22 Molecule (CD22))
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适用
- 人, 灵长类
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD22 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)
- 交叉反应 （详细）
- Species reactivity (tested):Human, non-human Primates
- 纯化方法
- Protein-A affinity chromatography
- 纯度
- > 95 % pure (by SDS-PAGE)
- 免疫原
- Raji Burkitt's lymphoma cell line
- 克隆位点
- MEM-01
- 亚型
- IgG1
- Top Product
- Discover our top product CD22 Primary Antibody
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anti-CD22 Molecule (CD22) (AA 621-725) antibody
CD22 适用: 人 FACS, WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 2H1C4 unconjugated
anti-CD22 Molecule (CD22) (AA 52-178) antibodyCD22 适用: 人 FACS, WB, ELISA, IHC, IF, Coat, StM 宿主: 小鼠 Monoclonal BLCAM-1795 unconjugated
anti-CD22 Molecule (CD22) (AA 621-725) antibodyCD22 适用: 人 FACS, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1A3A11 unconjugated
anti-CD22 Molecule (CD22) (AA 52-178) antibodyCD22 适用: 人 FACS, WB, ELISA, IHC, IF, Coat 宿主: 小鼠 Monoclonal BLCAM-1796 unconjugated
anti-CD22 Molecule (CD22) antibodyCD22 适用: 人, Non-Human Primate FACS, WB, IP 宿主: 小鼠 Monoclonal MEM-01 unconjugated
anti-CD22 Molecule (CD22) antibodyCD22 适用: 人, 小鼠, 大鼠, 猴, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4C3 unconjugated
anti-CD22 Molecule (CD22) antibody (PE)CD22 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-01 PE
anti-CD22 Molecule (CD22) antibody (FITC)CD22 适用: 人 FACS 宿主: 小鼠 Monoclonal IS7 FITC
anti-CD22 Molecule (CD22) (AA 52-178) antibodyCD22 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-022R unconjugated Recombinant Antibody
anti-CD22 Molecule (CD22) (AA 708-847) antibodyCD22 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Flow cytometry. Immunoprecipitation. Western blotting: Recommended dilution: 2-4 μg/mL, 120 min on vertical incubator Positive control: RAJI human lymphoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. - 有效期
- 12 months
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- 抗原
- CD22 (CD22 Molecule (CD22))
- 别名
- CD22 (CD22 产品)
- 别名
- A530093D23 antibody, Lyb-8 antibody, Lyb8 antibody, SIGLEC-2 antibody, SIGLEC2 antibody, siglec-2 antibody, CD22 antigen antibody, CD22 molecule antibody, Cd22 antibody, CD22 antibody
- 背景
- CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.Synonyms: B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, Leu-14, SIGLEC2, Sialic acid-binding Ig-like lectin 2, Siglec-2
- UniProt
- P20273
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