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CD38 抗体

This anti-CD38 antibody is a 小鼠 单克隆 antibody detecting CD38 in FACS, WB, IHC (fro) 和 IHC (p). Suitable for 人. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN120671
发货至: 中国

Quick Overview for CD38 抗体 (ABIN120671)

抗原

See all CD38 抗体
CD38

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD38 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

HIT2
  • 特异性

    The antibody reacts with CD38 (T10), a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes, it is an antigenic marker of lymphoid cells.

    纯化方法

    Protein-G Affinity Chromatography.

    免疫原

    Peripheral blood mononuclear cells of a patient suffering with CLL

    亚型

    IgG1
  • 应用备注

    Flow Cytometry. Western Blotting (non reducing conditions): 2 mg/mL. Positive control: RAJI human cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Boil for 5 min. Immunohistochemistry on frozen sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS, pH ~7.4, 15 mM Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf Life: one year from despatch.

    有效期

    12 months
  • Qu, Shen, Shen, Hu, Yang, Wang, Yan, Zhang: "Open-tubular gas chromatography using capillary coated with octadecylamine-capped gold nanoparticles." in: Analytica chimica acta, Vol. 609, Issue 1, pp. 76-81, (2008) (PubMed).

  • 抗原

    CD38

    背景

    CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.Synonyms: ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1, T10, cADPr hydrolase 1

    基因ID

    952

    UniProt

    P28907
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