KRT8 抗体
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北京 101111
Quick Overview for KRT8 抗体 (ABIN120663)
抗原
See all KRT8 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The antibody C-43 reacts with Cytokeratin 8 (52.5 kDa).
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纯化方法
- Affinity Chromatography on Protein A
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免疫原
- Cytoskeleton preparation from HeLa human cervix carcinoma cell line
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亚型
- IgG1
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应用备注
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Immunoprecipitation. Western Blot. Immunocytochemistry. Immunohistochemistry (Paraffin): 20 m g/mL. Positive tissue: prostate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. -
有效期
- 12 months
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: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).
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- KRT8 (Keratin 8 (KRT8))
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别名
- Cytokeratin 8
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背景
- Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.Synonyms: CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8
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分子量
- 52.5 kDa
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基因ID
- 3856
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UniProt
- P05787
抗原
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