NEFM 抗体
Quick Overview for NEFM 抗体 (ABIN120651)
抗原
See all NEFM 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The antibody reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
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纯化方法
- Affinity Chromatography on Protein A
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免疫原
- Pellet of porcine brain cold stable proteins after depolymerization of microtubules
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亚型
- IgG2a
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anti-Neurofilament Triplet M Protein (NEFM) (AA 779-916) antibody
NEFM 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1B6B12 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) antibodyNEFM 适用: 哺乳动物 WB, ICC, IHC (p) 宿主: 小鼠 Monoclonal NF-09 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 381-443) antibodyNEFM 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 2G12 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 101-200) antibodyNEFM 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 105-287) antibodyNEFM 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 200-300) antibodyNEFM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (C-Term) antibodyNEFM 适用: 大鼠 WB, IF 宿主: 小鸡 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (C-Term) antibodyNEFM 适用: 大鼠 WB, IF 宿主: 小鼠 Monoclonal 3H11 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (Internal Region) antibodyVerified NEFM 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) antibodyNEFM 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
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应用备注
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Immunohistochemistry on paraffin sections. Immunocytochemistry. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. -
有效期
- 12 months
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- NEFM (Neurofilament Triplet M Protein (NEFM))
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别名
- Neurofilament M (160 kD)
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背景
- Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons, the longest cell protrusions known in vertebrates. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights (NF-H - heavy chain, NF-M - medium chain and NF-L - light chain). The three NF subunits share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm.Synonyms: (Neuronal Marker), NEF3, NEFM, NF-M, Neurofilament 3, Neurofilament medium polypeptide
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UniProt
- Q4QRK6
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途径
- Brown Fat Cell Differentiation
抗原
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