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SIRPA 抗体

This anti-SIRPA antibody is a 小鼠 单克隆 antibody detecting SIRPA in WB, FACS, IHC (p) 和 IHC (fro). Suitable for 大鼠.
产品编号 ABIN120471
发货至: 中国

Quick Overview for SIRPA 抗体 (ABIN120471)

抗原

See all SIRPA 抗体
SIRPA (Signal-Regulatory Protein alpha (SIRPA))

适用

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大鼠

宿主

  • 95
  • 57
  • 8
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  • 1
小鼠

克隆类型

  • 106
  • 60
  • 1
单克隆

标记

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This SIRPA antibody is un-conjugated

应用范围

  • 84
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  • 9
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  • 1
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Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

OX-41
  • 特异性

    This anitbody recognises CD172a, also known as Signal regulatory protein (SIRP). MRC OX-41 binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a - CD47.

    纯化方法

    Affinity chromatography on Protein G

    免疫原

    Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with the NSO/U mouse myeloma cell line.

    亚型

    IgG2a
  • 应用备注

    Flow Cytometry: 1/100 - 1/200, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen and paraffin sections: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS, pH 7.4 containing 0.09 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    SIRPA (Signal-Regulatory Protein alpha (SIRPA))

    别名

    CD172a / SIRPA

    背景

    CD172a is expressed selectively by myeloid cells and by neurons.Synonyms: BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, MFR, MYD1, Macrophage fusion receptor, MyD-1 antigen, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, Signal-regulatory protein alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84

    基因ID

    25528

    NCBI登录号

    NP_037148

    UniProt

    P97710
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