Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)
特异性
This antibody recognises the beta chain of the rat CD8 cell surface antigen. This antibody is reported to block proliferative and cytotoxic responses of CD8 +ve effector cells in vitro.
纯化方法
Affinity chromatography on Protein G
免疫原
Rat/mouse T cell hybrids expressing CD8. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
CD8B
适用: Cow, 山羊, 绵羊, Water Buffalo
FACS
宿主: 小鼠
Monoclonal
CC58
PE
应用备注
Flow Cytometry: 1/100 - 1/200, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4 containing 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD8 is expressed on thymocytes and peripheral cytotoxic/suppressor cells. CD8b is not expressed by NK cells or by intestinal intraepithelial T cells.Synonyms: CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain