This antibody recognises the beta chain of the rat CD8 cell surface antigen. This antibody is reported to block proliferative and cytotoxic responses of CD8 +ve effector cells in vitro.
纯化方法
Affinity chromatography on Protein G
免疫原
Rat/mouse T cell hybrids expressing CD8. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Flow Cytometry: 1/100 - 1/200, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4 containing 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
CD8B
(CD8b Molecule (CD8B))
别名
CD8 beta
背景
CD8 is expressed on thymocytes and peripheral cytotoxic/suppressor cells. CD8b is not expressed by NK cells or by intestinal intraepithelial T cells.Synonyms: CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain