CD4 抗体 (Domain 1)
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- 抗原 See all CD4 抗体
- CD4
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抗原表位
- Domain 1
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD4 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)
- 纯化方法
- Affinity chromatography on Protein G
- 免疫原
- Rat Thymocyte Membrane Glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS-1 mouse myeloma cell line.
- 克隆位点
- W3-25
- 亚型
- IgG1
- Top Product
- Discover our top product CD4 Primary Antibody
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- 应用备注
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Flow cytometry (1/100 - 1/200): use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. (The product is routinely tested on rat splenocytes). Immunohistochemistry on frozen and paraffin sections. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-paraformaldehyde).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- CD4
- 别名
- CD4 (CD4 产品)
- 背景
- CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
- 基因ID
- 10116
- UniProt
- P05540
- 途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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