MADCAM1 抗体
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北京 101111
Quick Overview for MADCAM1 抗体 (ABIN120242)
抗原
See all MADCAM1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognizes Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM-1). Clone 17F5 recognises the Mucin domain of MAdCAM-1. This antibody does not block the function of MAdCAM-1.
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纯化方法
- Affinity Chromatography on Protein G.
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免疫原
- Recombinant soluble MAdCAM-1-fc fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
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亚型
- IgG1
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应用备注
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Flow Cytometry: 1/10- 1/100, use 10 μL of the suggested working dilution to label 106 cells in 100 μL. Immunohistochemistry on paraffin embedded sections: 1/50 - 1/100, This product does not require protein digestion prior to staining, This product does not require HIER prior to staining. ELISA. Western Blot: under reducing conditions, This antibody detects a band of approximately 60 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS containing 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
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别名
- MAdCAM1
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背景
- Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM-1) is a 60kD transmembrane protein that belongs to the immunoglobulin superfamily. MAdCAM-1 is strongly expressed on mucosal lymphoid tissue and high endothelial venules of Peyers patches. Human MAdCAM-1 interacts with the beta 7 integrin LPAM-1 (alpha4beta7), CD62L, and VLA-4 (alpha4beta1) and is involved in lymphocyte trafficking.Synonyms: MAdCAM-1, Mucosal addressin cell adhesion molecule 1, hMAdCAM-1
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基因ID
- 8174
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NCBI登录号
- NP_570116
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UniProt
- Q13477
抗原
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