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MADCAM1 抗体

The 小鼠 单克隆 anti-MADCAM1 antibody is suitable to detect MADCAM1 in samples from 人. It has been validated for WB, FACS, IHC (p) 和 EIA.
产品编号 ABIN120242
发货至: 中国
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for MADCAM1 抗体 (ABIN120242)

抗原

See all MADCAM1 抗体
MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This MADCAM1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

克隆位点

17F5
  • 特异性

    This antibody recognizes Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM-1). Clone 17F5 recognises the Mucin domain of MAdCAM-1. This antibody does not block the function of MAdCAM-1.

    纯化方法

    Affinity Chromatography on Protein G.

    免疫原

    Recombinant soluble MAdCAM-1-fc fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.

    亚型

    IgG1
  • 应用备注

    Flow Cytometry: 1/10- 1/100, use 10 μL of the suggested working dilution to label 106 cells in 100 μL. Immunohistochemistry on paraffin embedded sections: 1/50 - 1/100, This product does not require protein digestion prior to staining, This product does not require HIER prior to staining. ELISA. Western Blot: under reducing conditions, This antibody detects a band of approximately 60 kDa.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS containing 0.09 % Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

    别名

    MAdCAM1

    背景

    Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM-1) is a 60kD transmembrane protein that belongs to the immunoglobulin superfamily. MAdCAM-1 is strongly expressed on mucosal lymphoid tissue and high endothelial venules of Peyers patches. Human MAdCAM-1 interacts with the beta 7 integrin LPAM-1 (alpha4beta7), CD62L, and VLA-4 (alpha4beta1) and is involved in lymphocyte trafficking.Synonyms: MAdCAM-1, Mucosal addressin cell adhesion molecule 1, hMAdCAM-1

    基因ID

    8174

    NCBI登录号

    NP_570116

    UniProt

    Q13477
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