MADCAM1 抗体 (Biotin)
Quick Overview for MADCAM1 抗体 (Biotin) (ABIN120235)
抗原
See all MADCAM1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognises mucosal addressin cell adhesion molecule 1 (MAdCAM-1). Clone 314G8 does not recognise MAdCAM-1 in the gut lamina propria. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7. We recommend the use of SM2237LE for this purpose.
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纯化方法
- Affinity Chromatography on Protein G.
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免疫原
- Recombinant soluble MAdCAM-1-fc fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
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亚型
- IgG1
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anti-Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) antibody
MADCAM1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) antibodyMADCAM1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) (AA 173-190), (Middle Region) antibodyMADCAM1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) (AA 151-250) antibodyMADCAM1 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) (AA 19-317) antibodyMADCAM1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) (AA 85-180) antibodyMADCAM1 适用: 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) (AA 29-297) antibodyMADCAM1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry: (Neat-1/10): Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. ELISA: 1-10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
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别名
- MAdCAM1
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背景
- MAdCAM-1 is a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria.Synonyms: MAdCAM-1, Mucosal addressin cell adhesion molecule 1, hMAdCAM-1
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基因ID
- 8174
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NCBI登录号
- NP_570116
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UniProt
- Q13477
抗原
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