HLA-A2 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in FACS, IP, IHC (fro) 和 Func. Suitable for 人.
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Quick Overview for HLA-A2 抗体 (ABIN119575)
抗原
HLA-A2
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
克隆位点
BB7-2
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特异性
- This antibody recognises the human HLA-A2 histocompatibility antigen. The epitope recognised by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. BB7.2 may be used for the Flow cytometric detection of HLA-A2 expression and has also been used for Immunoaffinity purification of HLA-A2 Molecules. Functionally the antibody is reported to inhibit MHC restricted cellular cytotoxicity.
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纯化方法
- Affinity Chromatography on Protein G
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内毒素水平
- Less than 0.01EU/μg
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免疫原
- Papain solubilised HLA-A2. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
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亚型
- IgG2b
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anti-HLA-A2 antibody
适用: 人 FACS, IP 宿主: 小鼠 Monoclonal BB7-2 unconjugated
anti-HLA-A2 antibody (PE)适用: 人 FACS 宿主: 小鼠 Monoclonal BB7-2 PE
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应用备注
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Flow cytometry (10 μg/mL): use 10 μL of of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunoprecipitation. Functional assay (Inhibition of MHC resticted cytotoxicity): 5 - 10 μg/mL. Immunohistochemistry on frozen sections. Recommended positive control tissue: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS without stabilizer or preservative
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- HLA-A2
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别名
- HLA Class I A-2 alpha / HLA-A2
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物质类
- Influenza Protein
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背景
- Human leukocyte antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens, class I (HLA A, HLA B and HLA C) and class II (HLA D).Synonyms: A-2 alpha chain, HLA class I histocompatibility antigen, HLAA2, MHC class I antigen A-2
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基因ID
- 100507703
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NCBI登录号
- XP_003119274
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UniProt
- P01892
抗原
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