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CD3 抗体 (Cytoplasmic Domain)

CD3 适用: 人, 小鼠, 小鸡, Pig, 犬, 马 FACS, IHC (fro), IF, WB, IHC (p) 宿主: 大鼠 Monoclonal CD3-12 unconjugated
产品编号 ABIN119451
发货至: 中国
  • 抗原 See all CD3 抗体
    CD3
    抗原表位
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    适用
    • 189
    • 89
    • 23
    • 14
    • 14
    • 10
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    • 5
    • 3
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    • 1
    人, 小鼠, 小鸡, Pig, 犬, 马
    宿主
    • 215
    • 44
    • 14
    • 13
    • 10
    大鼠
    克隆类型
    • 284
    • 8
    单克隆
    标记
    • 88
    • 33
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    • 23
    • 22
    • 11
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    This CD3 antibody is un-conjugated
    应用范围
    • 266
    • 81
    • 54
    • 44
    • 44
    • 39
    • 27
    • 23
    • 9
    • 8
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    • 2
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    序列
    Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys
    特异性
    This antibody recognises a highly conserved epitope of the CD3 Molecule expressed by T lymphocytes. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species cross reactivity for the CD3 marker. (Jones et al. 1993, Kothlow et al. 2005).
    交叉反应 (详细)
    Species reactivity (expected):Mammals, Birds.
    Species reactivity (tested):Human, Mouse, Canine, Chicken, Equine, Porcine and Rhesus Monkey, Alpaca, Amazon Parrot, Duck, Harbour Porpoise, Koala, Raccoon, Sea Lion, Spotted Hyena.
    纯化方法
    Affinity Chromatography on Protein G
    免疫原
    Synthetic peptide sequence derived from Cytoplasmic epitope of CD3
    克隆位点
    CD3-12
    亚型
    IgG1
    Top Product
    Discover our top product CD3 Primary Antibody
  • 应用备注
    Flow cytometry (1/50-1/100, requires membrane permeabilisation). Immunohistochemistry on frozen sections and paraffin embedded tissues (1/1000). This product requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. 1 mM EDTA pH 8.0 is recommended for this purpose. Recommended positive control tissue: Tonsil. Western blotting.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    PBS, 0.09 % Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store the reconstituted antibody undiluted at 2-8 °C.
  • Hebel, Eisinger, Neumeier, Rein-Fischboeck, Pohl, Meier, Boettcher, Froehner, Adams, Liebisch, Krautbauer, Buechler: "Lipid abnormalities in alpha/beta2-syntrophin null mice are independent from ABCA1." in: Biochimica et biophysica acta, Vol. 1851, Issue 5, pp. 527-36, (2015) (PubMed).

    Hong, Puaux, Huang, Loumagne, Tow, Mackay, Kato, Prévost-Blondel, Avril, Nardin, Abastado: "Chemotherapy induces intratumoral expression of chemokines in cutaneous melanoma, favoring T-cell infiltration and tumor control." in: Cancer research, Vol. 71, Issue 22, pp. 6997-7009, (2011) (PubMed).

    Shrestha, Hashiguchi, Ito, Miura, Takenouchi, Oyama, Kawahara, Tancharoen, Ki-I, Arimura, Yoshinaga, Noma, Shrestha, Nitanda, Kitajima, Arimura, Sato, Sakamoto, Maruyama: "B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 4819-26, (2010) (PubMed).

  • 抗原
    CD3
    别名
    CD3 (CD3 产品)
    别名
    CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody
    背景
    T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
    基因ID
    916
    NCBI登录号
    NP_000724
    UniProt
    P07766
    途径
    TCR Signaling, Ubiquitin Proteasome Pathway
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