SNAP25 抗体
Quick Overview for SNAP25 抗体 (ABIN119027)
抗原
See all SNAP25 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody appears to recognize the pre-synaptic protein SNAP-25, an antigen of 26-27 kDa Molecular Weight. It will recognise SNAP-25 fusion protein from COS cells, but not the fusion protein from bacterial systems. Research studies have used this antibody to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy. (1,3,8)
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纯化方法
- Affinity Chromatography on Protein A.
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免疫原
- Crude Human synaptic immunoprecipitate.
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亚型
- IgG1
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anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 1-206) antibody
SNAP25 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 1-206) antibodySNAP25 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (C-Term) antibodyVerified SNAP25 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) antibodySNAP25 适用: 人 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 1E6 unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 166-206) antibodySNAP25 适用: 大鼠, 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) antibodySNAP25 适用: 人 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 1G2 unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 192-206) antibodySNAP25 适用: 人, 大鼠, 小鼠, 小鸡, 斑马鱼, Hamster, Goldfish WB, ELISA, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 31-130) antibodySNAP25 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4A3 unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (C-Term) antibodySNAP25 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) antibodySNAP25 适用: 人, 大鼠, 小鼠, Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1/5000-1/10000. ELISA. Immunohistochemistry on Paraffin Sections: 1/2000-1/5000, This product does not require protein digestion pre-treatment of paraffin sections, This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended positive control tissue: Brain.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))
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别名
- SNAP25
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背景
- SNAP25 is a soluble protein of 25 kDa which plays a key role in vesicle membrane fusion events with the plasma membrane. This protein was originally discovered to be enriched in subsets of neurons in the brain and displays a presynaptic pattern of expression. The membrane proteins SNAP25, synaptobrevin, and syntaxin form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. Functional interactions have been demonstrated to exist between SNAP-25, syntaxin, and the synaptic protein interaction site on voltage-sensitive L- and N-type calcium channels. SNAP-25 has been shown to be required for synaptic vesicle fusion with the plasma membrane during release of nerve growth factor (NGF). Regulated exocytosis of cortical granule secretion in fertilized eggs and membrane fusion events in neurons and endocrine cells is mediated by SNAP-25 in a calcium-dependent mechanism. SNAP25 protein levels have been shown to be elevated in prolactinoma and growth hormone (GH)/prolactin (PRL) tumor cells while reduced SNAP-25 protein expression has been observed between schizophrenic and normal hippocampal cells. Altered patterns of SNAP-25 expression have also been observed in the inferior temporal cortex and prefrontal association cortex between normal brains and brains from individuals affected with schizophrenia. SNAP25 accumulation due to cytoskeletal dysfunction is also observed in the swollen axons of the white matter of individuals with severe Alzheimer's dementia.Synonyms: RIC4, SNAP, SNAP-25, SUP, Super Protein, Synaptosomal-associated 25 kDa protein, Synaptosomal-associated protein 25, ric-4
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基因ID
- 6616
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NCBI登录号
- NP_003072
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UniProt
- P60880
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途径
- Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
抗原
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