Ubiquitin 抗体
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Quick Overview for Ubiquitin 抗体 (ABIN118999)
抗原
See all Ubiquitin (Ub) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognises ubiquitin.
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纯化方法
- Affinity chromatography on Protein G
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免疫原
- Ubiquitin from bovine erythrocytes conjugated to bovine gamma Globulin. Spleen cells from immunised BALB/c mouse were fused with cells of the mouse PAI myeloma cell line.
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亚型
- IgG1
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应用备注
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ELISA. Western Blot. Flow Cytometry: 1/10 - 1/50, Membrane permeabilisation is required for this application. Immunohistochemistry on frozen sections: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/500 - 1/1000, This product does not require protein digestion pre-treatment of paraffin sections, This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Human hypophysis.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Ubiquitin (Ub)
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别名
- Ubiquitin
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背景
- Synonyms: Polyubiquitin-B, UBB
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基因ID
- 7314
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UniProt
- P0CG47
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途径
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
抗原
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