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Ubiquitin 抗体

This anti-Ubiquitin antibody is a 小鼠 单克隆 antibody detecting Ubiquitin in WB, IHC (p), IHC (fro) 和 EIA. Suitable for 人 和 Cow.
产品编号 ABIN118999
发货至: 中国

Quick Overview for Ubiquitin 抗体 (ABIN118999)

抗原

See all Ubiquitin (Ub) 抗体
Ubiquitin (Ub)

适用

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人, Cow

宿主

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小鼠

克隆类型

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单克隆

标记

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This Ubiquitin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

克隆位点

1B4-UB
  • 特异性

    This antibody recognises ubiquitin.

    纯化方法

    Affinity chromatography on Protein G

    免疫原

    Ubiquitin from bovine erythrocytes conjugated to bovine gamma Globulin. Spleen cells from immunised BALB/c mouse were fused with cells of the mouse PAI myeloma cell line.

    亚型

    IgG1
  • 应用备注

    ELISA. Western Blot. Flow Cytometry: 1/10 - 1/50, Membrane permeabilisation is required for this application. Immunohistochemistry on frozen sections: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/500 - 1/1000, This product does not require protein digestion pre-treatment of paraffin sections, This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Human hypophysis.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS, pH 7.4 containing 0.09 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Ubiquitin (Ub)

    别名

    Ubiquitin

    背景

    Synonyms: Polyubiquitin-B, UBB

    基因ID

    7314

    UniProt

    P0CG47

    途径

    Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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