Poly-ADP-Ribose 抗体
PAR
适用: 人
WB, IHC (p), IHC (fro), IP
宿主: 小鼠
Monoclonal
10H
unconjugated
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- 抗原 See all Poly-ADP-Ribose (PAR) products
- Poly-ADP-Ribose (PAR)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Poly-ADP-Ribose antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
- 交叉反应 （详细）
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Species reactivity (expected):Broad.
Species reactivity (tested):Human. - 纯化方法
- Purified
- 免疫原
- Purified poly (ADP-ribose) polymer, 10-50 unit chain length.
- 克隆位点
- 10H
- 亚型
- IgG3
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- 应用备注
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Immunohistology on frozen sections: This product requires protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Immunoprecipitation. Western Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS, 50 % Glycerol
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Poly-ADP-Ribose (PAR)
- Abstract
- PAR 产品
- 别名
- 2310036I02Rik antibody, AW260507 antibody, Emv66 antibody, par antibody, AFG3-like AAA ATPase 2 antibody, Afg3l2 antibody
- 背景
- PADPR (Poly(ADP-ribose)) is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerases (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer PADPR, with chain lengths ranging from 2 to 300 residues, containing approximately 2 % branching in the chain. PADPR becomes attached to nuclear proteins, and to PARP itself (automodification). Under normal conditions, cells display low basal level of PADPR polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG).Synonyms: PADPR
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