Poly-ADP-Ribose 抗体
This 小鼠 单克隆 anti-Poly-ADP-Ribose antibody (Clone 10H) specifically detects Poly-ADP-Ribose in WB, IHC (p), IHC (fro) 和 IP. The antibody is reactive with 人 samples.
产品编号 ABIN118982
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Poly-ADP-Ribose 抗体 (ABIN118982)
抗原
Poly-ADP-Ribose (PAR)
适用
人
宿主
小鼠
克隆类型
单克隆
标记
This Poly-ADP-Ribose antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
克隆位点
10H
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交叉反应 （详细）
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Species reactivity (expected):Broad.
Species reactivity (tested):Human. -
纯化方法
- Purified
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免疫原
- Purified poly (ADP-ribose) polymer, 10-50 unit chain length.
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亚型
- IgG3
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应用备注
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Immunohistology on frozen sections: This product requires protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Immunoprecipitation. Western Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS, 50 % Glycerol
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Poly-ADP-Ribose (PAR)
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背景
- PADPR (Poly(ADP-ribose)) is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerases (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer PADPR, with chain lengths ranging from 2 to 300 residues, containing approximately 2 % branching in the chain. PADPR becomes attached to nuclear proteins, and to PARP itself (automodification). Under normal conditions, cells display low basal level of PADPR polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG).Synonyms: PADPR
抗原
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