F-Actin 抗体
This anti-F-Actin antibody is a 小鼠 单克隆 antibody detecting F-Actin in FACS, WB, IHC (fro) 和 EIA. Suitable for 人, 小鼠, 大鼠 和 兔.
Quick Overview for F-Actin 抗体 (ABIN118960)
抗原
F-Actin
适用
人, 小鼠, 大鼠, 兔
宿主
小鼠
克隆类型
单克隆
标记
This F-Actin antibody is un-conjugated
应用范围
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
克隆位点
NH3
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特异性
- This antibody recognizes Human Filamentous Actin (F-actin). The antibody binds to the N-terminal region of Actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. Clone NH3 is reported to recognise Actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule. Clone NH3 is also described to show reactivity with a 43 kDa polypeptide using cell lines U937 and HL-60 by SDS/PAGE and Immunoblotting.
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纯化方法
- Purified
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免疫原
- Human monocytes and U937 cell line. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
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亚型
- IgM
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anti-F-Actin (AA 1-50) antibody
适用: 人, 小鼠, 大鼠, 小鸡, 犬 FACS, WB, ELISA, IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-F-Actin (N-Term) antibody适用: 人, 小鼠, 大鼠, 兔 FACS, WB, IHC (fro), ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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Flow Cytometry (1:10). Immunohistochemistry on frozen and paraffin sections. This product may require protein digestion pretreatment (e.g. trypsin or pronase) or microwave antigen retrieval prior to staining of paraffin sections. ELISA (1:10) Western blotting (1:100 - 1:500)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- F-Actin
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别名
- Actin (F-Actin)
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背景
- Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. G-Actin (globular actin) with bound ATP can polymerise to form F-Actin (Filamentious Actin). Actin monomers spiral ariound the axis of the filament similar to a double helix. F-Actin may also undergo a process called treadmilling, in which filament length remains constant and actin monomers add at one end and dissociated at the other. Synonyms: Actin F type
抗原
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