Fibroblasts, Epithelial Cells 抗体
This 小鼠 单克隆 antibody specifically detects Fibroblasts, Epithelial Cells in FACS, IP 和 IHC (fro). It exhibits reactivity toward 人.It has been mentioned in 1 publication
(1 reference)
产品编号 ABIN118888
发货至:
中国
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Quick Overview for Fibroblasts, Epithelial Cells 抗体 (ABIN118888)
抗原
Fibroblasts, Epithelial Cells
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
克隆位点
D7-FIB
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特异性
- SM1214P recognises a 112 kDa molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS but resistant to trypsin, tunicamycin and collagenase. In immunohistochemical studies the antibody has also been found to bind to epithelium, myoepithelium, smooth muscle cells and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (Van Osch et al., 2001).
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无交叉反应
- 小鼠, 大鼠
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatography on Protein G
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免疫原
- Human foreskin Fibroblasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.
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亚型
- IgG2a
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应用备注
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Flow cytometry: use 10 μL of a 1:50 - 1:200 dilution to label 10^6 cells in 100 μL. This product is routinely tested in flow cytometry on the KG1 cell line. Immunohistochemistry on frozen tissues or cells (1:100): use ice-cold methanol for 5 min as fixative. Does not work on paraffin sections. Can also be used for immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS containing 0.09 % Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 9, pp. 5552-62, (2007) (PubMed).
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- Fibroblasts, Epithelial Cells
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别名
- Fibroblasts / Epithelial Cells
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背景
- A fibroblast is a connective-tissue cell of mesenchymal (somewhat undifferentiated) origin that secretes proteins from which the extracellular fibrillar matrix of connective tissue forms. Epithelial cells are cells that cover the surface of the body and line its cavities.Synonyms: Fibroblast Marker, Fibroblasten
抗原
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