CD58 抗体
Quick Overview for CD58 抗体 (ABIN118746)
抗原
See all CD58 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- BRIC5 was produced in response to erythrocytes. It reacts by Immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect haemagglutinin. The antigen on erythrocytes is Pronase sensitive. BRIC5 inhibits T cell rosetting.Aff. Const.:4 x 10e8 M-1
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纯化方法
- Affinity Chromatography on Protein G.
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免疫原
- Human erythrocytes.
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亚型
- IgG2a
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anti-CD58 (CD58) (AA 1-240) antibody
CD58 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 2D11-B10 unconjugated
anti-CD58 (CD58) (AA 1-214) antibodyCD58 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 31-130) antibodyCD58 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 40-220) antibodyCD58 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 1-241) antibodyCD58 适用: 人 WB, ELISA, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 2D11-B10 unconjugated
anti-CD58 (CD58) antibodyCD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 unconjugated
anti-CD58 (CD58) antibody (Biotin)CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 Biotin
anti-CD58 (CD58) antibody (APC)CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 APC
anti-CD58 (CD58) antibody (PE)CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 PE
anti-CD58 (CD58) (AA 29-215) antibodyCD58 适用: 人 FACS, WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 10^6 cells in 100 μL. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD58
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别名
- CD58 / LFA3
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背景
- CD58, or LFA-3, is a membrane glycoprotein of 55-70kD. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes. It is a member of the immunoglobulin superfamily of molecules. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 Molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.Synonyms: Ag3, LFA-3, Lymphocyte function-associated antigen 3
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基因ID
- 965
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NCBI登录号
- NP_001138294
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UniProt
- P19256
抗原
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