CD53 抗体
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- 抗原 See all CD53 抗体
- CD53
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD53 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- The antibody reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes, it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
- 纯化方法
- Protein-A affinity chromatography
- 纯度
- > 95 % pure (by SDS-PAGE)
- 免疫原
- Leukocytes of pacient suffering from a LGL-type leukemia
- 克隆位点
- MEM-53
- 亚型
- IgG1
- Top Product
- Discover our top product CD53 Primary Antibody
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anti-CD53 (CD53) antibody
CD53 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7A8D3 unconjugated
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 1H2C7 unconjugated
anti-CD53 (CD53) antibody (PE)CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-53 PE
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal MEM-53 unconjugated
anti-CD53 (CD53) (AA 100-180) antibodyCD53 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD53 (CD53) (Internal Region) antibodyCD53 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, WB, IF, IP, IHC 宿主: 兔 Chimeric HD77 unconjugated Recombinant Antibody
anti-CD53 (CD53) antibody (FITC)CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-53 FITC
anti-CD53 (CD53) (AA 65-160) antibodyCD53 适用: 人 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-CD53 (CD53) antibody (PE)CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal 4AC53-2 PE
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- 应用备注
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Flow Cytometry. Immunoprecipitation. Western Blotting. Immunohistochemistry on frozen sections. Suitable for discrimination of lymphomas from other tumors. Functional Application. The antibody induces activation of monocytes and B lymphocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- CD53
- 别名
- CD53 (CD53 产品)
- 背景
- CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.Synonyms: Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, TSPAN25, Tetraspanin-25
- 基因ID
- 963
- NCBI登录号
- NP_000551
- UniProt
- P19397
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