CD28 抗体 (PE)
Our Local Distributor
北京 101111
Quick Overview for CD28 抗体 (PE) (ABIN118584)
抗原
See all CD28 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- This antibody recognises the human CD28 cell surface antigen, a 44 kDa glycoprotein expressed by a subset of T lymphocytes. It has been reported that this clone also recognises an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2.
-
纯化方法
- Affinity chromatography on Protein G.
-
免疫原
- Human peripheral blood T-cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.
-
亚型
- IgG2b
-
-
anti-CD28 (CD28) (AA 1-120) antibody
CD28 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD28 (CD28) (AA 170-220) antibodyCD28 适用: 人, 小鼠 FACS, WB, IP, IHC, ICC 宿主: 兔 Monoclonal BLR200J unconjugated Recombinant Antibody
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, ICC, IHC (fro) 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibody (FITC)CD28 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CD28-2 FITC
anti-CD28 (CD28) (AA 19-140) antibodyCD28 适用: 人 IHC, ICC 宿主: 兔 Monoclonal BLR199J unconjugated Recombinant Antibody
anti-CD28 (CD28) (AA 19-152) antibodyCD28 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM63 unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, Func, ICC, IHC (fro) 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) (Internal Region) antibodyVerified CD28 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-CD28 (CD28) (C-Term) antibodyCD28 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人 FACS, IF, Func 宿主: 小鼠 Monoclonal CB28 unconjugated
-
-
应用备注
-
Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
溶解方式
- Restore with distilled water
-
缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- This product is photosensitive and should be protected from light
-
储存条件
- 4 °C
-
储存方法
- Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!
-
-
- CD28
-
别名
- CD28
-
背景
- Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95 % of CD4+ cells and 50 % of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 Molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
-
基因ID
- 940
-
NCBI登录号
- NP_006130
-
UniProt
- P10747
-
途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原
-
