CD4 抗体
Quick Overview for CD4 抗体 (ABIN118486)
抗原
See all CD4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognises CD4. Epitope mapping studies have shown that clone RPA-T4 recognises an epitope within domain 1 of the extracellular region of the CD4 Molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation (See Voehringer, D. et al. for details).
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纯化方法
- Affinity Chromatography on Protein G
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内毒素水平
- Low Endotoxin
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免疫原
- Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
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亚型
- IgG1
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应用备注
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Functional Assays. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunohistochemistry on frozen sections: 1/200 - 1/2000. Recommended Positive Control: Human lymphatic tissue
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, None, None
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Upon receipt, store undiluted (in aliquots) at -20 °C.
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- CD4
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别名
- CD4
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背景
- CD4 is a 55kD cell surface glycoprotein that is expressed primarily on a subpopulation of T lymphocytes and on peripheral blood monocytes and tissue macrophages.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
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基因ID
- 920
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NCBI登录号
- NP_000607
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UniProt
- P01730
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途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
抗原
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