CD3 抗体 (Biotin)
Quick Overview for CD3 抗体 (Biotin) (ABIN118477)
抗原
See all CD3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognises the CD3 cell surface antigen, expressed by all mature peripheral blood T lymphocytes.
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纯化方法
- Affinity Chromatography on Protein G
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免疫原
- Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line.
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亚型
- IgG1
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anti-CD3 (CD3) antibody
CD3 适用: 人, 小鼠, 大鼠 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), ICC, MS 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), Func, MS 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, Func, MS 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, MS 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal UCHT1 Biotin
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-57 Biotin
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PerCP
anti-CD3 (CD3) antibody (APC)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 APC
anti-CD3 (CD3) antibody (PE)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PE
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应用备注
- Flow Cytometry: Use 10 ul of neat-1/10 diluted antibody to label 10e6 cells or 100ul of lysed whole peripheral blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
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有效期
- 12 months
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- CD3
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别名
- CD3
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背景
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T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Alternate names: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4 -
基因ID
- 916
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NCBI登录号
- NP_000724
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UniProt
- P07766
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途径
- TCR Signaling, Ubiquitin Proteasome Pathway
抗原
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