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IL-4 抗体

The 大鼠 单克隆 anti-IL-4 antibody (Clone BVD4-1D11) (ABIN118404) specifically detects IL-4 in WB, FACS, EIA, IHC (fro) 和 ELISA (Capture). The antibody is reactive with 小鼠 samples.
产品编号 ABIN118404
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for IL-4 抗体 (ABIN118404)

抗原

See all IL-4 (IL4) 抗体
IL-4 (IL4) (Interleukin 4 (IL4))

适用

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小鼠

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大鼠

克隆类型

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单克隆

标记

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This IL-4 antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA (Capture)

克隆位点

BVD4-1D11
  • 特异性

    This antibody is specific for Mouse Interleukin-4 (IL-4). Clone BVD4-1D11 is reported to neutralise the effects of IL-4. We recommend the use of SM101A for functional studies.

    无交叉反应

    大鼠

    交叉反应 (详细)

    Species reactivity (tested):Mouse.

    纯化方法

    Affinity Chromatography on protein G

    免疫原

    Recombinant Mouse IL-4. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.

    亚型

    IgG1
  • 应用备注

    Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required. Immunohistochemistry on frozen sections. ELISA: 1 μg/mL - 5 μg/mL. Western Blot: 1 μg/mL - 5 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS containing 0.09 % Sodium Azide as preservative

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    IL-4 (IL4) (Interleukin 4 (IL4))

    别名

    Interleukin-4 / IL4

    背景

    Interleukin-4 is a potent lymphoid growth factor, which stimulates the growth and survival of certain B and T cells.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra

    基因ID

    16189

    NCBI登录号

    NP_067258

    UniProt

    P07750

    途径

    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
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