IL-4 抗体
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北京 101111
Quick Overview for IL-4 抗体 (ABIN118404)
抗原
See all IL-4 (IL4) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody is specific for Mouse Interleukin-4 (IL-4). Clone BVD4-1D11 is reported to neutralise the effects of IL-4. We recommend the use of SM101A for functional studies.
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无交叉反应
- 大鼠
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交叉反应 (详细)
- Species reactivity (tested):Mouse.
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纯化方法
- Affinity Chromatography on protein G
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免疫原
- Recombinant Mouse IL-4. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
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亚型
- IgG1
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应用备注
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Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required. Immunohistochemistry on frozen sections. ELISA: 1 μg/mL - 5 μg/mL. Western Blot: 1 μg/mL - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS containing 0.09 % Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- IL-4 (IL4) (Interleukin 4 (IL4))
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别名
- Interleukin-4 / IL4
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背景
- Interleukin-4 is a potent lymphoid growth factor, which stimulates the growth and survival of certain B and T cells.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
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基因ID
- 16189
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NCBI登录号
- NP_067258
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UniProt
- P07750
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
抗原
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