MHC Class II RT-1B 抗体
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北京 101111
Quick Overview for MHC Class II RT-1B 抗体 (ABIN118334)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Affinity Chromatography on Protein G
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免疫原
- Rat thymocyte membrane glycoprotein. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
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亚型
- IgG1
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应用备注
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Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunohistochemsitry on paraffin sections: 1/50 - 1/100, This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6, This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MHC Class II RT-1B
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别名
- MHC Class II RT1B
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背景
- Synonyms: HLA Class II, MHC Class 2 RT1B
抗原
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