Transferrin Receptor 抗体
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- 抗原 See all Transferrin Receptor (TFRC) 抗体
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This Transferrin Receptor antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- YTA 74.4 recognises the Mouse CD71 antigen. It is expressed on all dividing cells and can distinguish resting and activated T-cells. Immunoprecipitates a disulphide linked homodimer of 90 kDa by SDS PAGE. The antibody inhibits cell proliferation and the mixed lymphocyte response in vitro. Blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies (1,2).
- 纯化方法
- Protein G affinity purified Immunoglobulin fraction
- 免疫原
- Mouse Concanavilin A activated spleen cells.
- 克隆位点
- YTA 74-4
- 亚型
- IgG2a
- Top Product
- Discover our top product TFRC Primary Antibody
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- 应用备注
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Flow cytometry ( 10 μL of neat antibody to label 10^6 cells in 100 μL) Immunoprecipitation Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL IgG
- 缓冲液
- 0.09 % sodium azide as preservative and 1 % BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
- 别名
- CD71 / TFRC (TFRC 产品)
- 背景
- The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein, it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.Synonyms: TfR1, Transferrin receptor protein 1, p90
- 基因ID
- 22042
- NCBI登录号
- NP_035768
- UniProt
- Q62351
- 途径
- Transition Metal Ion Homeostasis
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