Biotin 抗体 (HRP)
Quick Overview for Biotin 抗体 (HRP) (ABIN118070)
抗原
适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Immunoaffinity chromatography
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免疫原
- Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
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anti-Biotin antibody
WB, ELISA, IHC, FACS, IF, FM 宿主: 小鼠 Monoclonal 4C7-D5 unconjugated
anti-Biotin antibodyWB, IHC, FACS, IF, ISt, CM 宿主: 小鼠 Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyIHC, ISt 宿主: 兔 Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibodyWB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Biotin antibody (DyLight 488)WB, FACS, IF, FM, FLISA, DB, MA 宿主: 山羊 Polyclonal DyLight 488
anti-Biotin antibodyELISA, FACS 宿主: 小鼠 Monoclonal SB58c unconjugated
anti-Biotin antibodyELISA, FACS, IP, MS 宿主: 小鼠 Monoclonal 1D4-C5 unconjugated
anti-Biotin antibody适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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应用备注
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Immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy andimmunohistochemistry as well as other peroxidase-antibody based enzymatic assaysrequiring lot-to-lot consistency. Recommended dilutions: Western blot 1: 500 - 1: 3,000. ELISA 1: 5,000 - 1: 25,000. Immunohistochemistry 1: 300 - 1: 1,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1.0 mL of deionized water or equivalent.
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Gentamicin Sulfate, 10 mg/mL BSA (IgG and Protease free)
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储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 4 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for four weeks at 4 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.
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- Biotin
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物质类
- Chemical
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背景
- Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
抗原
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