Thyroglobulin 抗体
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Quick Overview for Thyroglobulin 抗体 (ABIN118049)
抗原
See all Thyroglobulin (TG) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This protein A purified mouse monoclonal antibody reacts specifically with thyroglobulin in human tissues. The antibody recognizes a 330-kDa band corresponding to thyroglobulin. Cross reactivity with thyroglobulin from mouse, rat and dog will occur. Cross reactivity with thyroglobulin from other sources has not been determined.
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纯化方法
- Protein A chromatography.
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免疫原
- This protein A purified monoclonal antibody was produced by repeated immunizations with human thyroglobulin protein. Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma SP2/0 cells after immunization with thyroglobulin from human thyroid gland using conventional hybridoma technology.
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亚型
- IgG1
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应用备注
- Suitable for the detection of thyroglobulin in ELISA, Immunohistochemistry,Immunoblotting and Immunoprecipitation. For Immunohistochemistry, both frozen sections and formalin fixed, paraffin-embeddedtissue sections can be used without epitope retrieval or enzyme digestion. This antibody isspecific for the 330 kDa thyroglobulin protein. Thyroglobulin shows a cytoplasmiclocalization. Thyroid tissue can be used as a positive control. Generally a 1: 50 to 1: 200dilution is suitable for most applications. A 30 to 60 minute incubation time is usuallysufficient. However, depending upon the fixation conditions and the staining systememployed, optimal incubation should be determined by the user. Both frozen sections andformalin fixed, paraffin embedded tissues are recommended for preparations to use forImmunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.6 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 2-8°C.
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有效期
- 12 months
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- Thyroglobulin (TG)
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背景
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Thyroglobulin (Tg) is synthesized by the follicular epithelial cells of the thyroid and secreted from the thyroid gland with the stimulation of TSH and/or thyroid stimulating immunoglobulins. Thyroglobulin is a prognostic marker for Graves` disease. Thyroglobulin antibody has been useful in the positive identification of thyroid carcinomas of the papillary and follicular types.
Alternate names: TGN, Thyreoglobulin -
基因ID
- 7038
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NCBI登录号
- NP_003226
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UniProt
- P01266
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途径
- Thyroid Hormone Synthesis
抗原
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