Ferritin 抗体 (Heavy & Light Chain)
Our Local Distributor
北京 101111
Quick Overview for Ferritin 抗体 (Heavy & Light Chain) (ABIN118036)
抗原
See all Ferritin (FE) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- Heavy & Light Chain
-
纯化方法
- Delipidation and defibrination
-
免疫原
- Ferritin [Human Spleen]
-
-
anti-Ferritin (FE) antibody
FE 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ferritin (FE) antibodyKD Validated FE 适用: 人, 小鼠, 大鼠 WB, ICC, IF 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
-
-
应用备注
-
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayedagainst 1.0 μg of Ferritin [Human Spleen] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code R1364HRP and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes atroom temperature. A working dilution of 1: 10,000 to 1: 40,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
-
浓度
- 90.0 mg/mL (by Refractometry)
-
缓冲液
- 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2, 0.01 % (w/v) Sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store vial at 2-8 °C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
-
-
- Ferritin (FE)
-
别名
- Ferritin
-
背景
- Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits of two types, H (heavy, 21 kDa) and L (light, 19 kDa), and is capable of storing up to 4,500 atoms of ferric iron. Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely. Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria. As ferritin is an acute-phase reactant, it is often elevated during infection. Defects in ferritin proteins are associated with several neurodegenerative diseases.Synonyms: FTH, FTL, Ferritin H subunit, Ferritin L subunit
-
途径
- Transition Metal Ion Homeostasis
抗原
-
