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- 抗原 See all Fetuin A (AHSG) 抗体
- Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Fetuin A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.
- 纯化方法
- Multistep process.
- 免疫原
- This purified antibody was prepared from Rabbit serum after repeated immunizations with a recombinant Human Fetuin (Alpha-2-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein.
- Top Product
- Discover our top product AHSG Primary Antibody
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anti-alpha-2-HS-Glycoprotein (AHSG) (AA 200-240) antibody (Alexa Fluor 594)
AHSG 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal Alexa Fluor 594
anti-alpha-2-HS-Glycoprotein (AHSG) antibodyAHSG 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-HS-Glycoprotein (AHSG) (AA 19-250) antibodyAHSG 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-HS-Glycoprotein (AHSG) (AA 201-300) antibodyAHSG 适用: 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-HS-Glycoprotein (AHSG) (AA 247-276), (C-Term) antibodyAHSG 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB18674 unconjugated
anti-alpha-2-HS-Glycoprotein (AHSG) (AA 19-359) antibodyAHSG 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-HS-Glycoprotein (AHSG) (AA 19-367) antibodyAHSG 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 1D8 unconjugated
anti-alpha-2-HS-Glycoprotein (AHSG) antibodyAHSG 适用: 人 WB, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 2H2 unconjugated
anti-alpha-2-HS-Glycoprotein (AHSG) antibodyAHSG 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-HS-Glycoprotein (AHSG) (AA 1-367) antibodyAHSG 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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This purified polyclonal antibody reacts with human fetuin by ELISA (1/40,000-1/200,000)and Western blot (1/10,000-1/20,000). Although not tested, this antibody is likelyfunctional in Immunohistochemistry and Immunoprecipitation. A doublet band or slightlylower molecular weight band (see Figure 1, lane 1) may be visible by western blot due toproteolytic processing, variable glycosylation and/or phosphorylation. Proteolytic processing may include the removal of a 40 amino acid residue bridgingpeptide from the A and B chains of fetuin in vivo.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL of deionized water (or equivalent).
- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))
- 别名
- alpha-2-HS-Glycoprotein (AHSG 产品)
- 别名
- ahs antibody, a2hs antibody, hsga antibody, fetua antibody, wu:fb63g02 antibody, zgc:103687 antibody, AHSG antibody, MGC116429 antibody, A2HS antibody, AHS antibody, FETUA antibody, HSGA antibody, Aa2-066 antibody, pp63 antibody, alpha-2-HS-glycoprotein antibody, alpha 2-HS glycoprotein antibody, alpha-2-HS-glycoprotein 1 antibody, alpha-2-HS-glycoprotein L homeolog antibody, Cphamn1_1981 antibody, AHSG antibody, ahsg antibody, ahsg1 antibody, ahsg.L antibody, Ahsg antibody
- 背景
- Human Fetuin (2-Heremans-Schmid-glycoprotein or α2-HS-glycoprotein) is a major plasma glycoprotein predominantly synthesized in the liver. Human fetuin is named after its bovine homolog. Fetuins are found in most mammals. Human fetuin is a negative acute-phase protein, normal circulating levels in adults (300-600 μg/mL) fall significantly (30-50 % ) during injury and infection. The biological role of fetuin is unknown, although it has been implicated as an immunomodulator that can participate in stimulation of bacterial phagocytosis by neutrophils and promotion of endocytosis by mouse macrophages. Hepatocytes are the principal cell source of circulating fetuin, but it also is expressed by monocyte/macrophages. Fetuins occur in large amounts in blood and cerebrospinal fluid and accumulate to high concentrations in calcified bone. The fetuin promoter region has several potential interleukin 6-responsive elements, and its synthesis is down-regulated during injury and inflammation. Fetuin is an acidic glycoprotein with three Nlinked and three O-linked oligosaccharide chains, whose terminal sugar residues are rich in sialic acid (Nacetylneuraminic acid), contributing to its net negative charge. A role for fetuin as a carrier of bioactive molecules has been proposed based on observations that it binds and carries Ca2+ ion. Fetuin is implicated in bone remodeling, immune function and may play a role in tumor progression of certain cell types. Fetuin plays a role as an anti-inflammatory agent by suppressing the release of TNF from stimulated macrophages. Fetuin also interacts with members of the matrix metalloprotease family of zinc dependent secreted transmembrane proteins that degrade basement membranes and extracellular matrix components. The biological activity of fetuin is mediated through its direct interaction with other proteins. Fetuin down-regulates a number of receptor tyrosine kinase family members. A motif within fetuin has homology to the TGF-β receptor type II. Circulating human plasma fetuin is partly phosphorylated which implies that phosphorylated fetuin may have a physiological function in vivo. Human fetuin isolated from plasma is a two-chain molecule consisting of a A-chain of 322 amino acid residues (53 kDa) and a B-chain of 27 residues (5 kDa). The carboxy terminal 40 amino acids of the A chain constitute a bridging peptide that may be removed proteolytically in vivo. A single mRNA encodes both the A and B chains. The apparent molecular weight of intact human fetuin is 58 kDa.Synonyms: AHSG, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, FETUA, Fetuin A, PRO2743
- 基因ID
- 197
- NCBI登录号
- NP_001613
- UniProt
- P02765
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