NEK2 抗体 (AA 287-299)
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Quick Overview for NEK2 抗体 (AA 287-299) (ABIN117981)
抗原
See all NEK2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 287-299
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序列
- G-R-Q-L-G-E-P-E-K-S-Q-D-S Remarks: Human NEK2 is a protein kinase involved in mitotic regulation at the G2-M transition and/or at meiosis.
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特异性
- This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
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纯化方法
- Immunoaffinity chromatography.
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免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 287-299 of Human NEK2 protein.
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anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 148-397) antibody
NEK2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 331-445) antibodyNEK2 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 331-445) antibodyNEK2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2F9 unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 1-445) antibodyNEK2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 1-445) antibodyNEK2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) antibodyKD Validated NEK2 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB2085 unconjugated Recombinant Antibody
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 331-445) antibodyNEK2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C8 unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 282-295) antibodyNEK2 适用: 人 WB 宿主: 绵羊 Monoclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 396-426) antibodyNEK2 适用: 人 WB 宿主: 兔 Polyclonal RB2959 unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 331-445) antibodyNEK2 适用: 人 WB, IP, IHC (p), EIA 宿主: 小鼠 Monoclonal 2F6 unconjugated
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应用备注
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This affinity purified antibody has been tested for use in ELISA against the immunizingpeptide. Reactivity in other immunoassays is unknown. This product has been assayed byELISA against 0.1 μg of the immunizing peptide. A 1: 2,000 to 1: 8,000 dilution of theantibody is recommended for this assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.
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- NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))
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别名
- NEK2
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背景
- NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2. These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression. Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides. NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers.Synonyms: HSPK 21, NLK1, NimA-related protein kinase 2, Serine/threonine-protein kinase Nek2
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基因ID
- 4751
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NCBI登录号
- NP_001191111
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UniProt
- P51955
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途径
- M Phase
抗原
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