Cecropin B 抗体
The 兔 多克隆 anti-Cecropin B antibody is suitable to detect Cecropin B in samples from Bombyx mori. It has been validated for IP, WB 和 EIA.
产品编号 ABIN117971
发货至:
中国
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Quick Overview for Cecropin B 抗体 (ABIN117971)
抗原
Cecropin B
适用
Bombyx mori
宿主
兔
克隆类型
多克隆
标记
This Cecropin B antibody is un-conjugated
应用范围
Immunoprecipitation (IP), Western Blotting (WB), Enzyme Immunoassay (EIA)
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特异性
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using cecropin B coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified cecropin B. Reactivity against other forms of cecropins has not yet been determined.
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纯化方法
- Immunoaffinity chromatography
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免疫原
- This whole rabbit serum was prepared by repeated immunizations with a synthetically produced fulllength cecropin B corresponding to a modified sequence from that which is found in silkworm: Lys-Trp-Lys-Val-Phe-Lys-Lys-Ile-Glu-Lys-Val-Gly-Arg-Asn- Ile-Arg-Asn-Gly- Ile-Val-Lys-Ala-Gly-Pro-Ala-Ile-Ala-Val-Leu-Gly-Glu-Ala- Lys-Ala-Leu
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应用备注
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Anti-cecropin B is useful for the detection of cecropin B in various immunological assays. This reagent has specific value to study cecropins in transgenic plants. The antibody mayalso recognize naturally occurring cecropin A. Cecropin B has been modified in someinstances by substituting a single amino acid to render the molecule less susceptible toproteolytic cleavage. This reagent is suitable for the detection of cecropin B inImmunoblotting (1: 1000), Immunoprecipitation and ELISA experiments. A single 4400dalton band is detected in protein extracts.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.3 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.01 M Potassium Phosphate, 0.075 M Sodium Chloride, pH 7.2 with 50 % (v/v) Glycerol as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.
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- Cecropin B
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别名
- Cecropin-B
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背景
- Cecropins are produced by insects, particularly under conditions of infection. Cecropins are bioactive peptides that exhibit activities by interacting with membranes and forming transmembrane channels that allow the free flow of electrolytes, metabolites and water across the phospholipid bilayers. Cecropins A, B and D are close homologues consisting of 35-39 residues. They are found in the pupae of the cecropin moth, but related homologues named lepodopteran, bactericidin, moricin and sarcotoxin are produced by other insects.Synonyms: CECB1, Lepidopteran-A/B
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基因ID
- 693028
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NCBI登录号
- NP_001037460
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UniProt
- P04142
抗原
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