ANAPC11 抗体 (C-Term)
Quick Overview for ANAPC11 抗体 (C-Term) (ABIN117941)
抗原
See all ANAPC11 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 76-84, C-Term
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序列
- Human APC11 (84 aa 9.8 kDa): 1 mkvkikcwng vatwlwvand encgicrmaf ngccpdckvp gddcplvwgq cshcfhmhci 61 lkwlhaqqvq qhcpmcrqew kfke
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特异性
- This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This antibody reacts with human and mouse APC11. Cross reactivity may also occur with APC11 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and ROC2.
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纯化方法
- Delipidation and defibrination.
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免疫原
- This APC11 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 76-84 of Human APC11 (C-terminal) coupled to KLH.
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anti-Anaphase Promoting Complex Subunit 11 (ANAPC11) antibody
ANAPC11 适用: 人 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3F5 unconjugated
anti-Anaphase Promoting Complex Subunit 11 (ANAPC11) (AA 1-196) antibodyANAPC11 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 6F1H6 unconjugated
anti-Anaphase Promoting Complex Subunit 11 (ANAPC11) (AA 1-54) antibodyANAPC11 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 1B4-1A4 unconjugated
anti-Anaphase Promoting Complex Subunit 11 (ANAPC11) antibodyANAPC11 适用: 人 WB, IF 宿主: 小鼠 Monoclonal 1B5 unconjugated
anti-Anaphase Promoting Complex Subunit 11 (ANAPC11) (AA 56-94) antibodyANAPC11 适用: 人 WB 宿主: 兔 Polyclonal RB56253 unconjugated
anti-Anaphase Promoting Complex Subunit 11 (ANAPC11) (Internal Region) antibodyVerified ANAPC11 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Western blot (1: 500-1: 1,000). ELISA (1: 2,000-1: 10,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein andprotein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins (APC2) does occur. A 9.8 kDa bandcorresponding to human APC11 is detected. Most cell lines or tissues expressing APC11 canbe used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 85 mg/mL (by Refractometry)
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -80 °C for longer.
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- ANAPC11 (Anaphase Promoting Complex Subunit 11 (ANAPC11))
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别名
- APC11 / ANAPC11
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背景
- APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart, in moderate levels in brain, kidney, and liver, and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.Synonyms: Anaphase-promoting complex subunit 11, Cyclosome subunit 11, HSPC214, Hepatocellular carcinoma-associated RING finger protein
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基因ID
- 51529
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NCBI登录号
- NP_001002244
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UniProt
- Q9NYG5
抗原
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