MDC1 抗体 (AA 679-694)
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Quick Overview for MDC1 抗体 (AA 679-694) (ABIN117918)
抗原
See all MDC1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 679-694
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序列
- Peptide Sequence: G-G-T-K-D-S-E-D-N-Y-G-D-S-E-D-L
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特异性
- This is an affinity purified antibody produced by Immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
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纯化方法
- Immunoaffinity chromatography.
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免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 679-694 of Human MDC1 (mediator of DNA damage checkpoint protein 1). MDC1 is hyper-phosphorylated in an ATM- dependent manner.
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anti-Mdc1 (MDC1) antibody
MDC1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Mdc1 (MDC1) (AA 31-130) antibodyMDC1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mdc1 (MDC1) (N-Term) antibody (APC)MDC1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal P2B11 APC
anti-Mdc1 (MDC1) (N-Term) antibody (HRP)MDC1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal P2B11 HRP
anti-Mdc1 (MDC1) (N-Term) antibody (FITC)MDC1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal P2B11 FITC
anti-Mdc1 (MDC1) (N-Term) antibody (Atto 390)MDC1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal P2B11 Atto 390
anti-Mdc1 (MDC1) (N-Term) antibody (Atto 488)MDC1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal P2B11 Atto 488
anti-Mdc1 (MDC1) (N-Term) antibody (PerCP)MDC1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal P2B11 PerCP
anti-Mdc1 (MDC1) (N-Term) antibody (PE)MDC1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal P2B11 PE
anti-Mdc1 (MDC1) (N-Term) antibody (Atto 594)MDC1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal P2B11 Atto 594
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应用备注
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This affinity purified antibody has been tested for use in ELISA against the immunizingpeptide. Reactivity in other immunoassays is unknown. This product has been assayed by ELISA against 0.1 μg of the immunizing peptide. A1: 3,000 to 1: 12,000 dilution of the antibody is recommended for this assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
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- MDC1 (Mdc1 (MDC1))
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别名
- MDC1
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背景
- MDC1 (Mediator of DNA damage checkpoint protein 1) plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, both within S phase and G2/M. It is thought to act as a scaffold protein during the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A.X on S139. MDC1 is also involved in downstream events subsequent to the recruitment of these proteins. MDC1 has tandem repeat BRCT domains that are required for localisation to chromatin that flanks sites marked by S139 phosphorylated histone H2A.X. MDC1 is phosphorylated following exposure to ionising radiation (IR), ultraviolent radiation (UV), and hydroxyurea (HU). MDC1 is also phosphorylated during G2/M and during activation of the mitotic spindle checkpoint.Synonyms: KIAA0170, MDC1, Mediator of DNA damage checkpoint protein 1, NFBD1
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基因ID
- 9656
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NCBI登录号
- NP_055456
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UniProt
- Q14676
抗原
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