Cyanine Dyes 抗体 (Biotin)
This anti- antibody is a 绵羊 多克隆 antibody detecting in IF, EIA, FACS 和 IHC (fro). Suitable for .
Quick Overview for Cyanine Dyes 抗体 (Biotin) (ABIN117887)
抗原
Cyanine Dyes
宿主
绵羊
克隆类型
多克隆
标记
Biotin
应用范围
Immunofluorescence (IF), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
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产品特性
- Molar Ratio: 10-20 BAC molecule per sheep IgG molecule.
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纯化方法
- Immunoaffinity chromatography
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免疫原
- A mixture of Cy3 and Cy5 conjugated KLH
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应用备注
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Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assay using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended dilutions: This product has been assayed against 1.0 μg of Cyanine IgG in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 8,000 to 1: 40,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % Sodium Azide as preservative and 10 mg/mL BSA as stabilzer, IgG and Protease free.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Cyanine Dyes
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物质类
- Chemical
抗原
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